4OAG

Crystal structure of the cytosolic domain of mouse MiD51 bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.01
Spacegroup name	P 1 21 1
Unit cell lengths	62.068, 80.811, 65.376
Unit cell angles	90.00, 105.74, 90.00

Refinement procedure

Resolution	33.999 - 2.000
R-factor	0.1731
Rwork	0.171
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.148
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.705	34.705	2.040
High resolution limit [Å]	1.939	6.130	1.940
Rmerge		0.018	0.442
Number of reflections	44016
<I/σ(I)>	15.6	33.2	1.8
Completeness [%]	95.6	97.5	82.2
Redundancy	3.4	3.4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296.15	100 mM Bis-Tris, 100 mM lithium sulfate, 10 mM magnesiun chloride, 25% PEG 3350, 10 mM ADP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K