4OAF

Crystal structure of the cytosolic domain of mouse MiD51

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	91.075, 78.592, 102.310
Unit cell angles	90.00, 96.61, 90.00

Refinement procedure

Resolution	32.005 - 2.200
R-factor	0.2054
Rwork	0.204
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.141
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.296	39.296	1.970
High resolution limit [Å]	1.872	5.920	1.870
Rmerge		0.021	0.021
Number of reflections	110262
<I/σ(I)>	6.9	18.9	0.4
Completeness [%]	93.4	93.2	74.8
Redundancy	2.8	2.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296.15	100 mM HEPE, 700 mM Potassium sodium tartrate tetrahydrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K