4OAA
Crystal structure of E. coli lactose permease G46W,G262W bound to sugar
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115869 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.560, 121.940, 264.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.000 - 3.500 |
| R-factor | 0.2583 |
| Rwork | 0.257 |
| R-free | 0.29290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cfq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.899 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 3.200 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.110 | 0.194 |
| Number of reflections | 31737 | |
| <I/σ(I)> | 6.47 | 0.08 |
| Completeness [%] | 95.3 | 61.7 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 10mg/ml protein (20mM Hepes, .2% NG)and 5mM TDG against 1M NaCl, 0.05M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
