4O8Q

Crystal structure of bovine MHD domain of the COPI delta subunit at 2.15 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-28
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	42.092, 110.333, 145.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 2.150
R-factor	0.184
Rwork	0.182
R-free	0.22900
Structure solution method	MR-SAD
Starting model (for MR)	FROM INITIAL SAD EXPERIMENT
RMSD bond length	0.008
RMSD bond angle	1.159
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER ((phenix))
Refinement software	PHENIX ((phenix.refine: dev_1565))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.990
High resolution limit [Å]	1.960	1.960
Number of reflections	43300
<I/σ(I)>	28.2	2.7
Completeness [%]	88.0	77.9
Redundancy	2.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			293	0.2M Sodium citrate tribasic dehydrate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
2			293	0.4M NaCl, 0.1M Hepes 7.5, 18% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K