4O88
Crystal structure of a C-tagged Nuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 130 |
| Detector technology | PIXEL |
| Collection date | 2013-01-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.176 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.879, 77.455, 147.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.950 - 2.900 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.24660 |
| Structure solution method | Molecular refinement |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.065 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.950 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 16216 | |
| Completeness [%] | 98.8 | 94.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 100 mM Sodium citrate, 3 - 3.5 M Ammonium sulfate, 100 mM Magnesium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
