4O7X
Crystal structure of human ALKBH5 in complex with Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2012-03-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.093, 57.093, 145.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.780 |
| R-factor | 0.2154 |
| Rwork | 0.214 |
| R-free | 0.23762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nrm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.160 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 23830 | |
| Completeness [%] | 99.3 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 20% PEG 4K, 100mM CH3CO2NH4, 100mM Na-citrate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
