4O7Q
Crystal Structure of the F27G AIM2 Pyrin Domain Mutant and Similarities of its Self-association to DED/DED Interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.940, 37.950, 76.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.902 - 1.820 |
| R-factor | 0.1805 |
| Rwork | 0.178 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | combined model |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.828 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.870 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.057 | 0.670 |
| Number of reflections | 7526 | |
| <I/σ(I)> | 29.7 | 3.1 |
| Completeness [%] | 90.0 | 51 |
| Redundancy | 10.6 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 289 | 0.2 M CaCl2, 0.1 M Sodium Acetate, and 23 % PEG3350, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
