4O5P
Crystal structure of an uncharacterized protein from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.603, 81.078, 92.145 |
| Unit cell angles | 97.83, 89.96, 90.01 |
Refinement procedure
| Resolution | 28.298 - 2.001 |
| R-factor | 0.2258 |
| Rwork | 0.226 |
| R-free | 0.26930 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.199 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.212 |
| Number of reflections | 109609 | |
| <I/σ(I)> | 24.6 | 4.3 |
| Completeness [%] | 100.0 | 92.3 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.04M citric acid, 0.1M Bis-Tris propane, 17% pEG 3350 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
