4O5E
Structure of human DNA polymerase complexed with N7MG in the template base paired with incoming non-hydrolyzable TTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Detector technology | AREA DETECTOR |
| Collection date | 2013-10-19 |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.425, 78.920, 54.819 |
| Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.532 |
| R-factor | 0.2088 |
| Rwork | 0.205 |
| R-free | 0.27280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.532 |
| Number of reflections | 14806 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 14%-23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole, pH 7.5, VAPOR DIFFUSION, SITTING DROP |
