4O4L
Tubulin-Peloruside A-Epothilone A complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-02 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.400, 156.330, 180.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.100 - 2.200 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22400 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 404J |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.837 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.100 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.122 | 4.112 |
| Number of reflections | 149698 | |
| <I/σ(I)> | 15.9 | 0.75 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 13.6 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 293 | 6% PEG, 16% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1M MES/0.1M imidazole, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
