Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794, 0.97912 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 103.425, 103.425, 131.951 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 74.110 - 2.097 |
R-factor | 0.1662 |
Rwork | 0.165 |
R-free | 0.19502 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.568 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.110 | 2.140 |
High resolution limit [Å] | 2.097 | 2.097 |
Rmerge | 0.082 | 0.957 |
Number of reflections | 48245 | |
<I/σ(I)> | 36.6 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.5 | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.0M succinic acid, 0.1M Bis-Tris Propane, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289.0K |