4O34
Room temperature macromolecular serial crystallography using synchrotron radiation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 296 |
| Detector technology | PIXEL |
| Collection date | 2013-08-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.27 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.300, 79.300, 38.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.070 - 2.090 |
| R-factor | 0.18152 |
| Rwork | 0.176 |
| R-free | 0.23011 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.076 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.070 | 2.140 |
| High resolution limit [Å] | 1.700 | 2.090 |
| Number of reflections | 16081 | |
| <I/σ(I)> | 8.1 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1755 | 1281 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 3 | 293 | 14.7 % (w/v) NaCl, 22 % (w/v) PEG 8000, 500 mM acetate buffer pH 3, 100 mg/ml lysozyme, batch, temperature 293K |
