4O1W
Crystal Structure of Colwellia psychrerythraea cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-12 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.280, 45.043, 87.574 |
| Unit cell angles | 90.00, 90.85, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.000 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cno |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.527 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 19.950 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 34186 | |
| Completeness [%] | 99.1 | 92.2 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 30% PEG 4000, 0.2M lithium sulfate, 50mM Tris, 5% trehalose, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
