4O0H
Crystal structure of human L-asparaginase protein with covalently linked substrate L-asparagine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 59.651, 59.651, 298.517 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.830 - 1.970 |
| R-factor | 0.16881 |
| Rwork | 0.166 |
| R-free | 0.23139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GDV |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.655 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 38615 | |
| <I/σ(I)> | 9.16 | 2.05 |
| Completeness [%] | 91.9 | 80.5 |
| Redundancy | 3.36 | 3.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.2-2.5 M sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
