4NZG
Crystal Structure of the N-terminal domain of Moloney murine leukemia virus integrase, Northeast Structural Genomics Consortium Target OR3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.07500 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.659, 38.440, 135.275 |
Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
Resolution | 45.072 - 2.152 |
R-factor | 0.2359 |
Rwork | 0.234 |
R-free | 0.26540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nnq |
RMSD bond length | 0.005 |
RMSD bond angle | 0.795 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 135.220 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.081 | 0.605 |
Number of reflections | 24941 | |
<I/σ(I)> | 2.46 | |
Completeness [%] | 98.1 | 97 |
Redundancy | 6.7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution:1.0 M K2HPO4, 0.1 M NaAc, 0.05% Anapoe X-305, VAPOR DIFFUSION, HANGING DROP, temperature 295K |