4NY3
Human PTPA in complex with peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9687 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.670, 47.970, 87.180 |
| Unit cell angles | 84.41, 80.23, 88.23 |
Refinement procedure
| Resolution | 29.174 - 1.797 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.18550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.851 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 62666 | |
| Completeness [%] | 97.0 | 86.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 2 M (NH4)2SO4 and 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
