4NXU
Crystal structure of the cytosolic domain of human MiD51
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 1 |
Unit cell lengths | 72.573, 79.265, 79.375 |
Unit cell angles | 65.44, 84.18, 63.35 |
Refinement procedure
Resolution | 39.189 - 2.300 |
Rwork | 0.216 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.028 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.082 | 0.489 |
Number of reflections | 62135 | |
<I/σ(I)> | 10.8 | 2.7 |
Completeness [%] | 97.8 | |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 16% PEG 8000, 0.1M HEPES, 10% 2-propanol, 0.2M ammonium sulfate, 0.01M ADP, 0.01M manganese chloride , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |