4NXI
Rv2466c Mediates the Activation of TP053 To Kill Replicating and Non-replicating Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.060, 41.109, 66.900 |
| Unit cell angles | 99.26, 84.59, 102.83 |
Refinement procedure
| Resolution | 39.660 - 1.698 |
| R-factor | 0.1452 |
| Rwork | 0.143 |
| R-free | 0.17920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ime |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.289 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.664 | 1.800 |
| High resolution limit [Å] | 1.698 | 1.700 |
| Rmerge | 0.039 | 0.245 |
| Number of reflections | 36376 | |
| <I/σ(I)> | 12.8 | 2.7 |
| Completeness [%] | 80.24 | |
| Redundancy | 3.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1M TRIS pH 8.5, 0.20M MgCl2, 30% (w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
