4NWY
Crystal structure of the b' domain of human protein disulfide isomerase-like protein of the testis (PDILT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.977, 119.736, 33.291 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.19739 |
| Rwork | 0.195 |
| R-free | 0.23828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bj5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.043 | 0.211 |
| Number of reflections | 30080 | |
| <I/σ(I)> | 24.7 | 4.2 |
| Completeness [%] | 98.9 | 92.8 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 0.2 M sodium formate, 0.1 M Bicine pH 9.0, 18% PEG3350, 0.1 M cobaltous chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
