4NW4

Crystal structure of a DUF4822 family protein (EF0375) from Enterococcus faecalis V583 at 1.85 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-21
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 65
Unit cell lengths	61.379, 61.379, 160.389
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.731 - 1.850
R-factor	0.1512
Rwork	0.150
R-free	0.18150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.384
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.731	26.731	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge		0.013	0.013
Total number of observations		2526	14866
Number of reflections	29118
<I/σ(I)>	9.4	16.5	0.6
Completeness [%]	99.9	97.3	99.9
Redundancy	6.6	7.4	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	2.400M ammonium sulfate, 0.1M TRIS pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K