4NW3
Crystal structure of MLL CXXC domain in complex with a CpG DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28231 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.327, 40.225, 60.959 |
| Unit cell angles | 90.00, 93.51, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.820 |
| R-factor | 0.2206 |
| Rwork | 0.218 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3QMB AND 3QMG |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.326 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.920 |
| High resolution limit [Å] | 2.820 | 6.070 | 2.820 |
| Rmerge | 0.066 | 0.039 | 0.951 |
| Rmeas | 0.066 | ||
| Total number of observations | 30008 | ||
| Number of reflections | 4229 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 99.8 | 98 | 100 |
| Redundancy | 3.7 | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 20% PEG3350, 0.05 M sodium tartrate, VAPOR DIFFUSION, temperature 291K |
