4NW2

Tandem chromodomains of human CHD1 in complex with Influenza virus NS1 C-terminal tail trimethylated at K229

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	48.557, 92.248, 110.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.968 - 1.900
R-factor	0.2014
Rwork	0.199
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b2w
RMSD bond length	0.006
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	Aimless (0.2.12)
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.694	47.250	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.075	0.012	0.012
Total number of observations		2474	18620
Number of reflections	39818
<I/σ(I)>	14.5	36.4	1.7
Completeness [%]	100.0	98.8	100
Redundancy	3.8	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	10% PEG8000, 0.2M magnesium chloride, 0.1M Tris, pH 8.5, vapor diffusion, sitting drop, temperature 291K