4NV6
C212A mutant of Synechococcus VKOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-10-22 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 |
| Unit cell lengths | 139.563, 139.563, 68.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.683 - 4.190 |
| R-factor | 0.3022 |
| Rwork | 0.299 |
| R-free | 0.33110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.842 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PARROT |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.270 |
| High resolution limit [Å] | 4.190 | 4.190 |
| Rmerge | 0.111 | 0.949 |
| Number of reflections | 5732 | |
| <I/σ(I)> | 16.4 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | A 20mg/ml protein solution was mixed overnight with 15mg/ml DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), and 0.5% DDM. This mixture was crystallized with a buffer containing 11% PEG1500, 8% glycerol, 5% ethanol, 0.1M MgCl2, 0.1M NaCl, and 0.1M sodium cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
