4NTY

Cesium sites in the crystal structure of acid-sensing ion channel in complex with snake toxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.3780
Spacegroup name	H 3
Unit cell lengths	151.530, 151.530, 123.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.044 - 2.650
R-factor	0.2184
Rwork	0.216
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.092
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1402))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge		0.654
Number of reflections	31004
<I/σ(I)>		2.25
Completeness [%]	99.8	97.7
Redundancy		2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	100 mM sodium acetate, 6-9% PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K