4NTS
Apo structure of the catalytic subunit of cAMP-dependent protein kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Detector technology | CCD |
Collection date | 2011-12-08 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.960, 142.610, 63.160 |
Unit cell angles | 90.00, 103.65, 90.00 |
Refinement procedure
Resolution | 56.380 - 2.900 |
R-factor | 0.24675 |
Rwork | 0.244 |
R-free | 0.29185 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j3h |
RMSD bond length | 0.006 |
RMSD bond angle | 1.013 |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 61.380 |
High resolution limit [Å] | 2.900 |
Number of reflections | 19014 |
Completeness [%] | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277.15 | 8% MPD, 0.1 M Bicine, 150 mM Ammonium Acetate, 10 mM DTT, 9% methanol added to the well solution, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |