4NTD
Crystal structure of HlmI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.350, 96.350, 133.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.1471 |
| Rwork | 0.146 |
| R-free | 0.15938 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fk1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.259 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.056 | 0.498 |
| Number of reflections | 93867 | |
| <I/σ(I)> | 11.2 | 2.2 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 1.3M Na/K phosphate, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
