4NTC
Crystal structure of GliT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.790, 118.180, 69.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.18583 |
| Rwork | 0.184 |
| R-free | 0.22272 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fbs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.079 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.543 |
| Number of reflections | 51186 | |
| <I/σ(I)> | 9.8 | 2.3 |
| Completeness [%] | 94.5 | 97.6 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M tri-Na citrate, 20% PEG3000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
