4NST
Crystal structure of human Cdk12/Cyclin K in complex with ADP-aluminum fluoride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.720, 78.850, 91.490 |
| Unit cell angles | 104.04, 85.76, 101.46 |
Refinement procedure
| Resolution | 48.711 - 2.200 |
| R-factor | 0.1975 |
| Rwork | 0.195 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3blq 2i53 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.631 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 65263 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris, pH 6.5, 20.5% PEG 3350, 0.4 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
