4NS0
The C2A domain of Rabphilin 3A in complex with PI(4,5)P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-18 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.970, 38.910, 88.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.360 - 1.800 |
| R-factor | 0.18612 |
| Rwork | 0.184 |
| R-free | 0.22604 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2chd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | XFIT |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.360 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.780 |
| Rmerge | 0.131 | 0.720 |
| Number of reflections | 12421 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 94.2 | 96.9 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25mM Hepes, 20mM Ammonuim Sulphate, 25% PEG 4K, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
