4NRY
Crystal Structure of HIV-1 Neutralizing Antibody m66
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-19 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 98.501, 100.724, 166.848 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.840 - 3.140 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fab |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.746 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.240 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Rmerge | 0.136 | 0.521 |
| Number of reflections | 26613 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 91.3 | 50.3 |
| Redundancy | 6.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | 52.5% polyethylene glycol 400, 0.2 M calcium acetate, 0.1 M acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
