4NRQ

Crystal structure of human ALKBH5 in complex with pyridine-2,4-dicarboxylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	130
Detector technology	CCD
Collection date	2013-06-10
Detector	Bruker Platinum 135
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	56.767, 56.767, 144.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.22507
Rwork	0.224
R-free	0.24938
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nrm
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	8721
Completeness [%]	99.0	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	20% PEG 4K, 100mM CH3CO2NH4, 100mM Na-citrate , pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 289K