4NRM

Crystal structure of human ALKBH5 in complex with citrate and acetate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	130
Detector technology	CCD
Collection date	2011-11-10
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	56.726, 56.726, 146.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.170
R-factor	0.20501
Rwork	0.203
R-free	0.23215
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3btz
RMSD bond length	0.005
RMSD bond angle	1.335
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.170	2.170
Number of reflections	13344
Completeness [%]	99.0	91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	20% PEG 4K, 100mM CH3CO2NH4, 100mM Na-citrate , pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 289K