4NRL
Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2013-08-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872A |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 83.610, 128.400, 211.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.487 - 2.720 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.932 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: dev_1452)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 2.870 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.118 | 0.491 |
| Number of reflections | 59313 | |
| <I/σ(I)> | 13.7 | 4 |
| Completeness [%] | 95.9 | 87.1 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.1 M di-Sodium succinate 15% w/v PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
