4NRL

Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	200
Detector technology	CCD
Collection date	2013-08-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872A
Spacegroup name	P 21 2 21
Unit cell lengths	83.610, 128.400, 211.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.487 - 2.720
R-factor	0.1961
Rwork	0.194
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.932
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: dev_1452))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.910	2.870
High resolution limit [Å]	2.720	2.720
Rmerge	0.118	0.491
Number of reflections	59313
<I/σ(I)>	13.7	4
Completeness [%]	95.9	87.1
Redundancy	6.6	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	0.1 M di-Sodium succinate 15% w/v PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K