4NRJ
Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 2013-08-09 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 83.690, 128.600, 211.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.182 - 2.530 |
R-factor | 0.1883 |
Rwork | 0.187 |
R-free | 0.22150 |
Structure solution method | MIR |
RMSD bond length | 0.003 |
RMSD bond angle | 0.877 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: dev_1452)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.630 | 2.670 |
High resolution limit [Å] | 2.530 | 2.530 |
Rmerge | 0.114 | 0.483 |
Number of reflections | 75220 | |
<I/σ(I)> | 13.1 | 4.4 |
Completeness [%] | 97.9 | 93.9 |
Redundancy | 7.4 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.1 M Di-sodium succinate 15% w/v PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |