4NRI
Crystal Structure of a human Mms2/Ubc13 A122G mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-02-04 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.612, 78.187, 92.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.095 - 2.300 |
R-factor | 0.1881 |
Rwork | 0.186 |
R-free | 0.23520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7d |
RMSD bond length | 0.004 |
RMSD bond angle | 0.864 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.149 | 0.054 | 0.551 |
Number of reflections | 14213 | ||
<I/σ(I)> | 10 | ||
Completeness [%] | 96.2 | 88.3 | 89.8 |
Redundancy | 3.8 | 3.9 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 20% PEG 8000, 0.1 M sodium citrate, pH 6.8, vapor diffusion, sitting drop, temperature 298K |