4NRG

Crystal Structure of a human Mms2/Ubc13 D118G mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-02-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	44.272, 73.616, 92.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.136 - 1.950
R-factor	0.1799
Rwork	0.178
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j7d
RMSD bond length	0.005
RMSD bond angle	0.860
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Rmerge	0.090	0.041	0.439
Number of reflections	21609
<I/σ(I)>	15.7
Completeness [%]	95.3	97.1	89.3
Redundancy	4.2	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	298	20% PEG 8000, 0.1 M sodium citrate, pH 6.8, vapor diffusion, sitting drop, temperature 298K