4NRE
The structure of human 15-lipoxygenase-2 with a substrate mimic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 155.794, 155.794, 263.219 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.949 - 2.630 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.721 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.550 | 2.770 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 34599 | |
| Completeness [%] | 99.7 | 69.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295.15 | 1M (NH4)2SO4, 0.1M BisTris pH 5.5, 5% Glycerol, 24mM C8E4, 40mM CaCl2 soak for 30 min, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
