4NQX
Crystal Structure of HLA A*0101 in complex with NP44-S7N, an 9-mer influenza epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 264.610, 81.411, 140.290 |
| Unit cell angles | 90.00, 121.82, 90.00 |
Refinement procedure
| Resolution | 45.750 - 2.000 |
| R-factor | 0.2707 |
| Rwork | 0.270 |
| R-free | 0.28270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nqv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 119.207 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 165625 |
| <I/σ(I)> | 14 |
| Completeness [%] | 97.1 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 277 | 0.2M MgCl, 0.1M Na-citrate pH 5.6, 15-20% PEG 6K, EVAPORATION, temperature 277K |
