4NPJ

Extended-Synaptotagmin 2, C2A- and C2B-domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.38592
Spacegroup name	P 43
Unit cell lengths	62.300, 62.300, 226.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.892 - 2.101
R-factor	0.2019
Rwork	0.200
R-free	0.23880
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.904
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.067	0.041	0.501
Number of reflections	48539
<I/σ(I)>	13.1		1.46
Completeness [%]	97.0	99.3	73.8
Redundancy	5.5	6.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.9	293	2.25 M LiCl, 0.1 M sodium acetate, 0.125 M NaCl, 2.5% glycerol, 25% ethylene glycol, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.9	293	2.25 M LiCl, 0.1 M sodium acetate, 0.125 M NaCl, 2.5% glycerol, 25% ethylene glycol, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K