4NP8

Structure of an amyloid forming peptide VQIVYK from the second repeat region of tau (alternate polymorph)

Replaces:  3FQP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	28.635, 4.880, 35.807
Unit cell angles	90.00, 110.47, 90.00

Refinement procedure

Resolution	16.780 - 1.510
R-factor	0.1791
Rwork	0.177
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2on9
RMSD bond length	0.012
RMSD bond angle	1.399
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	1.620
High resolution limit [Å]	1.500	1.500
Rmerge	0.160	0.376
Number of reflections	825
<I/σ(I)>	8.1	4.7
Completeness [%]	94.4	82.1
Redundancy	4.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	reservoir contained 14% iso-Propanol, 0.07M HEPES-Na pH 7.5, 0.14M Sodium Citrate, and 30% Glycerol, vapor diffusion, hanging drop, temperature 298K