4NO4

Crystal Structure of Galectin-1 L11A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-08-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	112.520, 193.830, 108.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.969 - 1.399
R-factor	0.1882
Rwork	0.188
R-free	0.19710
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.479
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX (dev_1593)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.400	1.400
Number of reflections	443997
Completeness [%]	98.6	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	292	PEG 4K, ammonium sulfate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K