4NLV
Poliovirus Polymerase - G289A/C290F Loop Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-23 |
Detector | NOIR-1 |
Spacegroup name | P 65 |
Unit cell lengths | 128.530, 128.530, 111.623 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.755 - 2.300 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | d*TREK (9.4L) |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.750 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.075 | 0.358 |
Number of reflections | 41646 | |
<I/σ(I)> | 11.9 | 3.7 |
Completeness [%] | 99.6 | 100 |
Redundancy | 6.57 | 6.44 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 289 | Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid, 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |