4NLU

Poliovirus Polymerase - G289A Loop Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-06
Detector	NOIR-1
Wavelength(s)	1.000
Spacegroup name	P 65
Unit cell lengths	129.093, 129.093, 112.466
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.225
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ra6
Data reduction software	d*TREK (9.4LDz)
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.260	42.260	2.020
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.061	0.028	0.497
Total number of observations		53929	52214
Number of reflections	77401
<I/σ(I)>	11	33.5	2
Completeness [%]	100.0	100	100
Redundancy	6.8	6.85	6.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid, and 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K