4NLR
Poliovirus Polymerase - C290S Loop Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-06 |
| Detector | NOIR-1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 129.466, 129.466, 112.357 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ra6 |
| Data reduction software | d*TREK (9.4LDz) |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.380 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.074 | 0.482 |
| Number of reflections | 91000 | |
| <I/σ(I)> | 9.8 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.76 | 6.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 289 | Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid and 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
