4NLQ

Poliovirus Polymerase - C290F Loop Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-21
Detector	NOIR-1
Spacegroup name	P 65
Unit cell lengths	128.735, 128.735, 111.923
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.222
Rwork	0.222
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ra6
Data reduction software	d*TREK (9.4L)
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.348
Number of reflections	46552
<I/σ(I)>	12.8	4.3
Completeness [%]	99.6	100
Redundancy	7.41	7.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	289	Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid and 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K