4NLP

Poliovirus Polymerase - C290V Loop Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-25
Detector	NOIR-1
Spacegroup name	P 65
Unit cell lengths	128.710, 128.710, 111.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.222
Rwork	0.222
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ra6
Data reduction software	d*TREK (9.4L)
Data scaling software	d*TREK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.072	0.392
Number of reflections	53309
<I/σ(I)>	15.3	4.5
Completeness [%]	100.0	100
Redundancy	6.77	6.55

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	289	Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid, and 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K