4NLM
1.18 Angstrom resolution crystal structure of uncharacterized protein lmo1340 from Listeria monocytogenes EGD-e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.224, 69.990, 52.905 |
| Unit cell angles | 90.00, 95.61, 90.00 |
Refinement procedure
| Resolution | 24.210 - 1.180 |
| R-factor | 0.14678 |
| Rwork | 0.145 |
| R-free | 0.18116 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.594 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.200 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.076 | 0.459 |
| Number of reflections | 112400 | |
| <I/σ(I)> | 31.7 | 2.33 |
| Completeness [%] | 95.9 | 97.4 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | protein - 7.5 mg/mL in 10 mM Tris-HCl, pH 8.3, 0.5 M NaCl 5 mM BME, Crystallization - The PACT Suite (#D2: 0.1 M MMT buffer pH 5, 25 % (w/v) PEG1500, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
