4NLA

Structure of the central NEAT domain, N2, of the listerial Hbp2 protein, apo form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950, 0.9686, 1.5418
Spacegroup name	I 2 3
Unit cell lengths	89.300, 89.300, 89.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.140 - 2.700
R-factor	0.258
Rwork	0.254
R-free	0.29300
Structure solution method	MAD
Starting model (for MR)	3sz6
RMSD bond length	0.008
RMSD bond angle	1.020
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.140	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.052	0.471
Number of reflections	3370
<I/σ(I)>	35.3	6.3
Completeness [%]	100.0	100
Redundancy	21.4	22.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.4	298	100 mM sodium citrate tribasic dihydrate, 200 mM potassium sodium tartrate tetrahydrate, 2.0 M ammonium sulfate, pH 5.4, vapor diffusion, temperature 298K, EVAPORATION