4NL4

PriA Helicase Bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.008
Spacegroup name	P 32
Unit cell lengths	85.433, 85.433, 111.682
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.140 - 2.650
R-factor	0.21957
Rwork	0.218
R-free	0.25401
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.996
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Number of reflections	26617
<I/σ(I)>	23.78	2.34
Completeness [%]	100.0	100
Redundancy	7.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	10 mM Tris-HCl, pH 8.0, 5% MPD, 6% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K