4NKO
Crystal structure of engineered anti-EE scFv antibody fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.586, 104.917, 284.413 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.200 - 3.496 |
R-factor | 0.3102 |
Rwork | 0.308 |
R-free | 0.34410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ktr |
RMSD bond length | 0.004 |
RMSD bond angle | 1.249 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.200 | 3.620 |
High resolution limit [Å] | 3.490 | 3.490 |
Number of reflections | 11089 | |
<I/σ(I)> | 12 | 2.97 |
Completeness [%] | 93.1 | 95.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298.15 | Crystals of 3D5/His_683 (8mg/ml in HBS) were grown at room temperature with 0.2M KI, 0.001M Guanidinium HCl, 18% PEG 8000. These crystals appeared in 2-3 days., VAPOR DIFFUSION, SITTING DROP, temperature 298.15K |